How to imbinarize Cell nucleus

How can I just imbinarize dark purple Cell nucleus ? How can I detect Cell nucleus?

Answer 1

[Pratyush Roy]

Hi,

Firstly you can convert the RGB image to a grayscake image using the method rg2gray/ After this, you can use imbinarize with a threshold for converting grayscale image to binary image. The code snippet below illustrates how to obtain the cell nucleus:

cell_img = imread("cell_nucleus.png");
cell_img_gray = rgb2gray(cell_img);
BW = imbinarize(cell_img_gray,0.35); % The threshold is set to a low value of 0.35, so that only the dark purple pixels are obtained as black. 
imshow(BW)

Hope this helps!