Tracking a segmented line in grayscale images

Hi guys

I Need to trace a line following the profile of alternated dark-light ( D-L)structures to calculate these lines' roughness. I have a stack of (D-L)*n profiles and I want to draw a line at the middle of L layers, following the profile. I Aldo Need to store line data for further analysis.

Any suggestion Is warmly welcome.

Cheers

[darova]

Can you show the image?

[KALYAN ACHARJYA]

Marco's Comment moved here

Hi Darova,

thanks for the reply.

Here it is. This image is not that good but it is the only i currently have with me and it can work as an example. I need to track a single line for each bright section (or dark section, it is not important which). This line is not straight but should follow the section profile, with asperities. These data should be also stored for further analysis.

Answer 1

[KALYAN ACHARJYA]

One way

grayImage=rgb2gray(imread('image_track.png'));
bw_im=imbinarize(grayImage,0.6);
subplot(121),imshow(bw_im);
bw_im=bwareaopen(bw_im,20);
bw_im=~bwareaopen(~bw_im,20);
row_data=find(all(bw_im==1,2))
grayImage(row_data,:)=255;
subplot(122),,imshow(grayImage);

You can modify more to get the desired results