Counting silk worm eggs or fish eggs

Hi,

I am working on counting the no of eggs laid by silkworm or fish eggs.

I am doing that with image segmantaion technique with all the filters and noise removal but i am not getting best accurate output. I have to make sure that syatem takes inly eggs in to consideration but not the background letters and distrbances.

Can anyone please help in this ? Do you have any good techniques to solve this issue?

The sample pic is attached

[KALYAN ACHARJYA]

In fact I love these segmentation questions, although in RHS side has some challenges to get accurate results due to blurring/overlap.

[javadhi srinuvas]

yes. kalyan sir.

I expected answer from you sir. I have read your blogs which were very informative.

I am facing this issue too. Kinldy help me if possible

[Mangala Nageshwari]

Can any one please share me source code for this counting fish eggs to my gmail [e-mail]?

[Image Analyst]

@Mangala Nageshwari, please start your own question and upload your own photo. Place your eggs on a contrasting background, like black velvet, or a vividly colored paper. We'll put it in Answers so everyone can benefit from it.

Answer 1

[KALYAN ACHARJYA]

You can try several approaches to see how much accuracy can be stated only after the results.

One Way:

hsv_im=rgb2hsv(im_data);
threshold=.... % around 0.58 to 6
worm_im=hsv_im(:,:,3)>threshold;

Nest steps Morpho operation,

• Remove the largest opject (Outer Boundary)
• Remove all tiny pixels
• Apply Morpho imerode function (To disconnect the connected worm)
• Be care ful on previous steps, it might be remove smaller worm pixel data
• Counts the total object in the image

Note:The accuracy can be stated only after the results.

:)

[javadhi srinuvas]

Thank you sir.

There are 541 eggs on manual counting

[KALYAN ACHARJYA]

Have you tried the above approach?

[Vívian Helena]

I know almost nothing of matlab, but recently on my lab we've been counting fish eggs. Can u help me with a code for that? I'll need some detail since I dont know much of the language. thanks in advance in case ur able to help

[Image Analyst]

@Vívian Helena Sure, we could try to help. Start your own new question after doing the links below.

To learn fundamental concepts, invest 2 hours of your time here:

MATLAB Academy - Free 2 hour training

See my Image Segmentation Tutorial in my File Exchange:

Image Analyst's File Exchange

It's a generic, general purpose demo of how to threshold an image to find blobs, and then measure things about the blobs, and extract certain blobs based on their areas or diameters.