Creating an acquisition video using micromanager matlab core

Hello Everyone,

I am currently trying to create a GUI for Micromanager to control a microscope. I am trying to create an acquisition but am having trouble doing so. All I want the gui to do is create an acquisition file with the video or pictures that were taken, and also show a live feed in a separate figure. When I run my code below, the acquisition is running and thats about it. I have been trying to fix this for a couple of days but am making no progress. Your help will be greatly appreciated

            import mmcorej.*;
            mmc = CMMCore;
            mmc.loadSystemConfiguration('C:/Program Files/Micro-Manager-1.4/MMConfig_demo.cfg');            

            mmc.prepareSequenceAcquisition('Camera')
            mmc.startSequenceAcquisition(NumShots, Ms.*MultipleFactor , false)
            Sequencing = mmc.isSequenceRunning()

              while Sequencing == 1                         %mmc.isSequencingRunning() outputs 1 to indicate that it is sequencing
                Sequencing == mmc.isSequenceRunning()
                img = mmc.getLastImage();                 %Retrieves last image taken
                width = mmc.getImageWidth();
                height = mmc.getImageHeight();

                if mmc.getBytesPerPixel==2                 %Not sure exactly what this does but is shown in the matlab code
                    pixelType='uint16';
                else
                    pixelType='uint8';
                end

                img_final =typecast(img,pixelType);        %Formats the picture so that live video is adjusted properly
                img_final =reshape(img_final, [width, height]);
                img_final =transpose(img_final);

                %display image
                fig3 = figure(3);
                imshow(img_final);
                drawnow;

                if Sequencing == 0
                    close(fig3);
                end

            end

[Sebastian Bech-Terkilsen]

Hi Sukhraj, do you still need help?

[barath V]

Hii iam also facing a similar pblm,a reply to this question will be greatly beneficial.

Answer 1

[Karsten Schulz]

fig3 = figure(3); ?? (Unrecognized function or variable 'fig3'.)