V =
According to your description, at some stage of your procedure, the right-hand side of your ODE system is available as a symbolic vector depending on the state variables y. Here, you can easily compute the Jacobian in symbolic form and later on, substitute your fixed points and compute the eigenvalues as shown below.
syms y(t)
eqn = diff(y,2) + y^2 == 4 - diff(y);
[V,S] = odeToVectorField(eqn)
% Convert the Y[i] in odeToVectorField output V to yi
V_c = feval(symengine,'evalAt',V,'Y=[y1,y2]')
% Evaluate jacobian with respect to yi
J = jacobian(V_c, sym('y', [1 2]))
s = symvar(J)
Jnum = double(subs(J,s(1),2))
eig(Jnum)