Bioinformatics Toolbox - 'blastall' is not recognized as an internal or external command

I am receiving the following error when I try to run my local blast:

Error using blastlocal (line 294) BLASTALL produced the following error(s): 'blastall' is not recognized as an internal or external command, operable program or batch file.

This is from a piece of code that compiles a list of potential target sequences (500+ different 23 bp sequences) and blasts them against my genome database. output = blastlocal('InputQuery',data,'Program','blastn','Database', ... 'C:\Users\Brian\Genomics\blast\db\Nga_genome');

Answer 1

[Star Strider]

I can’t find ‘blastall’ other than references in a PubMed search. Guessing here because I don’t do genome research, but be sure you downloaded it and all the necessary associated files.

Also, be sure you downloaded it to a directory on your MATLAB Search Path. If you created a new directory for it, you need to be sure MATLAB knows where to look for it. See the documentation for details.

[Brian Vogler]

I have added the blast folder to the search path.

Answer 2

[Arthur Goldsipe]

Have you installed the BLAST+ executables? As stated on the blastlocal reference page:

"To use the blastlocal function, you must have a local copy of the NCBI blastall executable file (version 2.2.17) available from your system. You can download the blastall executable file by accessing BLAST+ executables, then clicking the download link under the blast column for your platform. Run the downloaded executable and configure it for your system."

[Brian Vogler]

I have installed the BLAST+ executables.