TIFF from ImageJ not loading correctly in R2024b (worked in R2022b)

Hi,

I’ve been using MATLAB to analyze 3D confocal images saved as TIFF files. My process is to get the original images from NIS-Elements, then open them in ImageJ to crop just the droplet region, and save that as a TIFF. In MATLAB R2022b, this TIFF input would allow the program to do a full 3D reconstruction of the droplet and calculate the data I need.

But now that I’m using R2024b, the same TIFF files don’t load properly. The 3D reconstruction step stops and doesn’t work, and I get no data even when I test it using the exact same TIFF files and inputs that worked previously.

I’m wondering if MATLAB R2024b handles TIFF files differently than R2022b? Are there specific settings I should use when saving TIFFs from ImageJ to make sure they work with the latest MATLAB? Or would this be entirely a different problem?

Thanks!

[Image Analyst]

You forgot to attach any file(s). Is it a single multi-page TIFF file, or a bunch of separate 2-D images? How are you reading it in? Are you using imread(), or the special TiffReader function? Do you get any warnings or errors? What exactly does "get no data" mean? Does it halt abruptly with an error? Does it process normally but all your results vectors (measurements) are null or all zeros or just gibberish?

You know, you can crop and mask images in MATLAB. If that's all you're using ImageJ for, then consider doing it all in MATLAB.

If you have any more questions, then attach your data and code to read it in with the paperclip icon after you read this:

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