attempt to change the axis of an axes limits remove the original image

Hello, I created GUI with several axis and pushing buttons. I loaded binary image to specific axes (let call it axes1). now i would like to change the image axis so it will be tight on the image and than plot on the image afterwards.

now i've been noticed that if i tried to change the image axis the image remove (the axes area turns gray). this is my code:

axes(handles.axes1)
imageAxes1 = imshow(temp_struct.binary_image);
axis([xmin xmax ymin ymax]);
hold on

and from some reason it doesn't work. there are any specific rule for changing axis in GUI? by the way i am using the guide for building the gui.

Thanks.

[Image Analyst]

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